• Cyclosporin Variants and Mitochondrial Pore Activity

  2026-09-11

  The reference study combines NMR spectroscopy, molecular dynamics, and mitochondrial swelling assays to explain why closely related cyclosporin variants differ in mitochondrial permeability transition pore activity. Its central finding is that greater peptide-backbone flexibility is associated with activity, whereas the more rigid Cyclosporin E is inactive under the tested conditions.

• Protease Inhibitor Cocktail: MS-SAFE Extraction Guide

  2026-09-11

  Protease Inhibitor Cocktail (MS-SAFE, 50X in DMSO) helps limit endogenous proteolysis during cell and tissue protein extraction, including workflows prepared for mass spectrometry. It should be used as a protease-focused reagent; phosphatase control, metalloproteinase inhibition, and compatibility with DMSO-sensitive assays require separate validation.

• Haloprogin: Evidence from a 1970 Topical Antifungal Study

  2026-09-10

  The 1970 study by Harrison and colleagues established Haloprogin as a topical antifungal with dermatophyte activity comparable to tolnaftate, while also demonstrating activity against Candida species and selected Gram-positive bacteria. Its combined serial-dilution, fungicidal, comparator, serum-effect, and guinea pig experiments provide a useful framework for interpreting topical antimicrobial performance and designing follow-up infection studies.

• FAISL-FAK Proteolysis in Triple-Negative Breast Cancer

  2026-09-10

  The reference study identifies FAISL as a long noncoding RNA that stabilizes focal adhesion kinase by preventing Calpain 2-mediated proteolysis, thereby promoting aggressive triple-negative breast cancer phenotypes. Its combination of transcriptomic discovery, mechanistic interaction studies, functional assays, and nanoparticle-mediated silencing provides a framework for studying FAK regulation beyond kinase activity alone.

• Deracoxib–Doxorubicin Protection in Canine Mammary Cells

  2026-09-09

  The 2017 reference study examined whether Deracoxib could reduce doxorubicin-associated injury in cultured normal canine mammary epithelial cells. Its combination of MTT viability testing, flow-cytometric apoptosis analysis, and Griess nitrite measurement linked cellular protection to reduced apoptosis and modulation of nitric oxide production, while also defining important limits for translational interpretation.

• Rapamycin (Sirolimus): mTOR Workflow Guide

  2026-09-09

  Use Rapamycin as a chemically defined mTOR perturbation for lipid autophagy, signaling, immunology, and disease-model experiments. This practical guide translates a salmon-cell multi-omics study into dose planning, assay selection, and troubleshooting strategies for reproducible bench workflows.

• METTL14–DHRS4-AS1 Axis in Ulcerative Colitis

  2026-09-08

  The 2024 Cell Biology and Toxicology study identifies METTL14 as a protective regulator of ulcerative colitis through m6A control of the lncRNA DHRS4-AS1 and its downstream miR-206/A3AR axis. Its combined cell and DSS-colitis experiments connect RNA methylation, epithelial injury, apoptosis, and NF-κB-associated inflammation, while also defining important limits for translating the mechanism into therapeutic research.

• Ginger–5-HT3 Receptor Docking: Implications for CINV

  2026-09-08

  The reference study used molecular docking and GRID analysis to map how gingerols, shogaols, and related compounds may interact with orthosteric and proposed allosteric sites on the murine 5-HT3 receptor. Its results provide a mechanistic hypothesis for ginger-derived 5-HT3 modulation in chemotherapy-induced nausea and vomiting, while also defining the experimental limits of structure-based affinity predictions.

• Dextran sulfate sodium salt in colitis models

  2026-09-07

  Dextran sulfate sodium salt provides a practical, adjustable route to model epithelial injury, inflammation, and mucosal repair in mice. This guide connects DSS exposure design with GPR35–KLF5 biology, assay selection, and troubleshooting for ulcerative colitis research.

• ISRIB (trans-isomer) in Memory-Forgetting Models

  2026-09-07

  ISRIB (trans-isomer) connects mechanistic ER stress research with inflammation-associated memory-loss experiments by restoring translation downstream of eIF2α phosphorylation. This guide translates recent mouse findings into practical cell, apoptosis, and recognition-memory workflows while emphasizing controls, timing, and troubleshooting.

• Electrostatic Sorting in α-Synuclein Condensates

  2026-09-05

  A 2025 Journal of Biological Chemistry study shows that α-synuclein condensates possess a strongly negative electrostatic potential that governs how labeled proteins and small molecules partition into them. The work combines charge-tuned fluorophores, zeta-potential measurements, and engineered intracellular probes, providing a framework for interpreting fluorescence-based condensate assays.

• Dronedarone (Multaq) Research Workflows

  2026-09-04

  Build reproducible Dronedarone (Multaq) workflows for automated patch clamp, atrial fibrillation treatment research, and comparative cardiac pharmacology. The guide emphasizes solvent control, KCa2 assay interpretation, and troubleshooting when a multi-target antiarrhythmic agent produces little direct KCa2 signal.

• Aneugen Molecular Mechanism Assay: 27-Chemical Proof

  2026-09-04

  Bernacki and colleagues developed a tiered flow-cytometric strategy that not only detects chemical-induced genotoxicity but also distinguishes major aneugenic mechanisms involving tubulin or mitotic kinases. Using 27 reference chemicals, the study combined biomarker profiling, fluorescent Taxol responses, hierarchical clustering, and neural-network classification to support mechanism-oriented interpretation of chromosome missegregation.

• Sodium Citrate: A Reagent Risk Map for SERS

  2026-09-03

  Sodium citrate can influence pH, metal-ion availability, protein behavior, and nanoparticle interfaces, but its role must not be inferred beyond the evidence. This article maps when citrate is useful, when it may confound SERS workflows, and how the cited polymer pen lithography study changes assay-design decisions.

• Toremifene Citrate: Evidence and Clinical Context

  2026-09-03

  The reference article presents toremifene citrate as a clinically useful nonsteroidal antiestrogen and oral selective estrogen receptor modulator for postmenopausal patients with advanced hormone-responsive breast cancer. Its main contribution is a practice-oriented synthesis connecting estrogen receptor pharmacology with comparative efficacy, pharmacokinetics, adverse-effect surveillance, drug interactions, and nursing management.

7897 records 1/527 page Next 12345 下5页 Last page